Sterility:Article Title: Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER 50 mL Conical Centrifuge Tubes Genesee Scientific Cat # 28-108 25 mL Wobble-not Serological Pipet Vistalab Cat# 4090-0025 10 mL Serological Pipets Sterile Genesee Scientific Cat# 12-104C 5 mL Serological Pipets Sterile Genesee Scientific Cat# 12-102C 1.7 mL Microtubes Genesee Scientific Cat# 24-282S 1000 μL Reach Olympus Premium Barrier Tips Genesee Scientific Cat# 24-430C 200 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2069-05-HR-HID 20 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2149P-05-HR 10 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2139-05-HR FastWells Incubation Chambers Electron Microscopy Sciences Cat# 70325-50 Medium used mTeSR plus Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL mTeSR plus with ROCK inhibitor Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL 100 mM Y27632 5 μM 25 μL Basal medium Final concentration Volume Advanced DMEM/F-12 N/A 500 mL GlutaMAX Supplement 100X 1X 5 mL L-Ascorbic acid phosphate 50 mg/mL 60 μg/mL 600 μL Penicillin-Streptomycin 100X 0.4X 2mL S1 medium Final concentration Volume Basal medium N/A 10 mL CHIR99021 6 μM 3 μL S2 medium Final concentration Volume Basal medium N/A 500 mL EGF Human 20 μL 10 ng/mL FGF 2 Human (147 a.a.) 250 μL 50 ng/mL VEGF human 250 μL 50 ng/mL STAR Protocols 7, 104294, March 20, 2026 5 Note: Once prepared, store at 4 ◦ C for 4 weeks.
Transferring:Article Title: Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER 50 mL Conical Centrifuge Tubes Genesee Scientific Cat # 28-108 25 mL Wobble-not Serological Pipet Vistalab Cat# 4090-0025 10 mL Serological Pipets Sterile Genesee Scientific Cat# 12-104C 5 mL Serological Pipets Sterile Genesee Scientific Cat# 12-102C 1.7 mL Microtubes Genesee Scientific Cat# 24-282S 1000 μL Reach Olympus Premium Barrier Tips Genesee Scientific Cat# 24-430C 200 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2069-05-HR-HID 20 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2149P-05-HR 10 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2139-05-HR FastWells Incubation Chambers Electron Microscopy Sciences Cat# 70325-50 Medium used mTeSR plus Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL mTeSR plus with ROCK inhibitor Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL 100 mM Y27632 5 μM 25 μL Basal medium Final concentration Volume Advanced DMEM/F-12 N/A 500 mL GlutaMAX Supplement 100X 1X 5 mL L-Ascorbic acid phosphate 50 mg/mL 60 μg/mL 600 μL Penicillin-Streptomycin 100X 0.4X 2mL S1 medium Final concentration Volume Basal medium N/A 10 mL CHIR99021 6 μM 3 μL S2 medium Final concentration Volume Basal medium N/A 500 mL EGF Human 20 μL 10 ng/mL FGF 2 Human (147 a.a.) 250 μL 50 ng/mL VEGF human 250 μL 50 ng/mL STAR Protocols 7, 104294, March 20, 2026 5 Note: Once prepared, store at 4 ◦ C for 4 weeks.
Incubation:Article Title: Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER 50 mL Conical Centrifuge Tubes Genesee Scientific Cat # 28-108 25 mL Wobble-not Serological Pipet Vistalab Cat# 4090-0025 10 mL Serological Pipets Sterile Genesee Scientific Cat# 12-104C 5 mL Serological Pipets Sterile Genesee Scientific Cat# 12-102C 1.7 mL Microtubes Genesee Scientific Cat# 24-282S 1000 μL Reach Olympus Premium Barrier Tips Genesee Scientific Cat# 24-430C 200 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2069-05-HR-HID 20 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2149P-05-HR 10 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2139-05-HR FastWells Incubation Chambers Electron Microscopy Sciences Cat# 70325-50 Medium used mTeSR plus Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL mTeSR plus with ROCK inhibitor Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL 100 mM Y27632 5 μM 25 μL Basal medium Final concentration Volume Advanced DMEM/F-12 N/A 500 mL GlutaMAX Supplement 100X 1X 5 mL L-Ascorbic acid phosphate 50 mg/mL 60 μg/mL 600 μL Penicillin-Streptomycin 100X 0.4X 2mL S1 medium Final concentration Volume Basal medium N/A 10 mL CHIR99021 6 μM 3 μL S2 medium Final concentration Volume Basal medium N/A 500 mL EGF Human 20 μL 10 ng/mL FGF 2 Human (147 a.a.) 250 μL 50 ng/mL VEGF human 250 μL 50 ng/mL STAR Protocols 7, 104294, March 20, 2026 5 Note: Once prepared, store at 4 ◦ C for 4 weeks.
Electron Microscopy:Article Title: Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER 50 mL Conical Centrifuge Tubes Genesee Scientific Cat # 28-108 25 mL Wobble-not Serological Pipet Vistalab Cat# 4090-0025 10 mL Serological Pipets Sterile Genesee Scientific Cat# 12-104C 5 mL Serological Pipets Sterile Genesee Scientific Cat# 12-102C 1.7 mL Microtubes Genesee Scientific Cat# 24-282S 1000 μL Reach Olympus Premium Barrier Tips Genesee Scientific Cat# 24-430C 200 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2069-05-HR-HID 20 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2149P-05-HR 10 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2139-05-HR FastWells Incubation Chambers Electron Microscopy Sciences Cat# 70325-50 Medium used mTeSR plus Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL mTeSR plus with ROCK inhibitor Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL 100 mM Y27632 5 μM 25 μL Basal medium Final concentration Volume Advanced DMEM/F-12 N/A 500 mL GlutaMAX Supplement 100X 1X 5 mL L-Ascorbic acid phosphate 50 mg/mL 60 μg/mL 600 μL Penicillin-Streptomycin 100X 0.4X 2mL S1 medium Final concentration Volume Basal medium N/A 10 mL CHIR99021 6 μM 3 μL S2 medium Final concentration Volume Basal medium N/A 500 mL EGF Human 20 μL 10 ng/mL FGF 2 Human (147 a.a.) 250 μL 50 ng/mL VEGF human 250 μL 50 ng/mL STAR Protocols 7, 104294, March 20, 2026 5 Note: Once prepared, store at 4 ◦ C for 4 weeks.
Concentration Assay:Article Title: Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER 50 mL Conical Centrifuge Tubes Genesee Scientific Cat # 28-108 25 mL Wobble-not Serological Pipet Vistalab Cat# 4090-0025 10 mL Serological Pipets Sterile Genesee Scientific Cat# 12-104C 5 mL Serological Pipets Sterile Genesee Scientific Cat# 12-102C 1.7 mL Microtubes Genesee Scientific Cat# 24-282S 1000 μL Reach Olympus Premium Barrier Tips Genesee Scientific Cat# 24-430C 200 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2069-05-HR-HID 20 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2149P-05-HR 10 μL Barrier Pipette Tips Thermo Fisher Scientific Cat# 2139-05-HR FastWells Incubation Chambers Electron Microscopy Sciences Cat# 70325-50 Medium used mTeSR plus Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL mTeSR plus with ROCK inhibitor Final concentration Volume mTeSR 1 Basal Medium N/A 400 mL mTeSR 1 5X Supplement 1X 100 mL 100 mM Y27632 5 μM 25 μL Basal medium Final concentration Volume Advanced DMEM/F-12 N/A 500 mL GlutaMAX Supplement 100X 1X 5 mL L-Ascorbic acid phosphate 50 mg/mL 60 μg/mL 600 μL Penicillin-Streptomycin 100X 0.4X 2mL S1 medium Final concentration Volume Basal medium N/A 10 mL CHIR99021 6 μM 3 μL S2 medium Final concentration Volume Basal medium N/A 500 mL EGF Human 20 μL 10 ng/mL FGF 2 Human (147 a.a.) 250 μL 50 ng/mL VEGF human 250 μL 50 ng/mL STAR Protocols 7, 104294, March 20, 2026 5 Note: Once prepared, store at 4 ◦ C for 4 weeks.
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